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KMID : 0380019950100010089
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Korean Journal of Biotechnology and Bioengineering
1995 Volume.10 No. 1 p.89 ~ p.95
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Molecular Approaches for Cloning of Important Higher Plant Genes
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Rose, Alna B
Last, Robert L.
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Abstract
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An Arabidopsis thaliana gene encoding phosphoribosyl anthranilate transferase is shown to be the gene that is defective in blue fluorescent trp 1 mutant plants. This gene, named PATl, coding region is homologous to those for the phosphoribosyl anthranilate transferase from many microorganisms. This is due to a defect in tryptophan biosynthesis that leads to an accumulation of anthranilate, a fluorescent intermediate in the tryptophan pathway. PATl is a single-copy gene that complements all of the visible phenotypes of the different trpl mutants. Experiments to determine the regulation of the PAT1 gene are in progress. The wild-type PAT1 promoter and several promoter deletions of PAT1 gene have been transformed into Arabidopsis tryptophan mutants. These constructs might identify promoter elements that control this patterns. We have isolated the homozygous lines in T, seeds and analyzed the protein levels using PAT antibody and PAT protein level increased two fold in pHS107. Finally, the potential of using PAT1 as a selectable marker or visible reporter of gene expression is being explored.
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